Epimedin C, icariin, icaritin dirhamnoside, baohuoside I, rutin, isoquercitrin, neohesperidin, glucohesperetin, naringin, prunin, myricetrin, myricetin, vitexin, dioscin, zingiberensis newsaponin, ginsenoside Rg2, and ginsenoside Re were all purchased from Sichuan Weikeqi Biotechnology Co., Ltd (China).

The standards of oleanolic acid, ursolic acid, stigmasterol, β-Sitosterol, and the internal standard danshensu (IS1) were purchased from National Institute for Food and Drug Control (Beijing, China). The standards of coniferin, ferulic acid, and the internal standard icariin (IS2) were supplied from Sichuan Weikeqi Biological Technology Co., Ltd. (Si chuan, China). All standards, which the purity was more than 98.0%, were suitable for UHPLC–MS/MS analysis.

Standard compounds,epimedin A, epimedin B, epimedin C, icariin and baohuoside I, were obtained from the Sichuan Weikeqi Biological Technology Co. Ltd(Chengdu, China). Analytical‐grade formic acid and anhydrous ethanolwere obtained from the Nanjing Chemical Reagent Co. Ltd (Jiangsu, China).