Acetonitrile and methanol (HPLC grade, >=99.9%) were supplied by Merck ltd. Formic acid was obtained from Sigma-Aldrich. Ultra pure water was purified using the Milli-Q system (Bedford, USA). The reference compounds of rhynchophylline (lot number: wkq18051803), verbascoside (lot number: 120524), linderane (lot number: wkq18031303), geniposidic acid (lot number: wkq18040312), coclaurine (lot number: wkq18036198) were supplied by Sichuan Weikeqi- Biotech Co., Ltd., nootkatone were obtained from Chengdu Chroma- Biotechnology Co., Ltd., rutin (lot number: 100080-201811), echinacoside (lot number: 111670-201907), quercetin (lot number:081-9003), and paeoniflorin (lot number:110736-201943) were obtained from National Institutes for Food and Drug Control.